Sample Collection Guidelines

Proper sample collection is critical for accurate pathogen detection. We have provided guidelines to help you collect samples correctly.

Before You Begin

Clean hands and sterile equipment are essential for accurate results. Contaminated samples may yield false positives or inconclusive results. Always wear clean gloves and sanitise collection tools between samples.

Plant Material Sample Collection Overview

Plant material samples are the most common sample type for detecting plant pathogens including viroids, fungi, and bacteria. Fresh, properly collected plant tissue provides the best results for molecular testing.

Sample Size

3-5 leaves per sample

Storage

Cool (4°C), dry conditions

Shelf Life

48-72 hours if refrigerated

Step-by-Step Collection Process

Prepare Your Equipment

Gather clean, sealable plastic bags or containers, clean scissors or pruning shears, disposable gloves, and a permanent marker for labelling.

Sterilise cutting tools between plants (eg. with 70% isopropyl alcohol)

Use new gloves for each sample to prevent cross-contamination

Pre-label bags before collecting to avoid confusion

Select the Right Material

Select material that best represent the condition you want to test for.

Avoid dead or heavily damaged tissue

Include leaves and material from a range of plant regions

Root material may be sampled as well where required

Collect the Sample

Using clean scissors or shears, cut the material from the selected plant. Handle gently to minimise bruising and tissue damage.

Remove sample material with clean and sterile implements

Avoid touching the material surfaces with bare hands

Work quickly to prevent wilting

Take note of the location of the sampled materials for future analysis and reporting

Package the Sample

Place the collected material in a clean, sealable plastic bag. Gently press out excess air before sealing. Avoid crushing or folding the material.

Include a slightly damp (not wet) paper towel to maintain humidity

Do not add water directly to the bag

Ensure the bag is properly sealed

Label and Store

Clearly label each sample with the date, plant identifier, location, and any relevant notes about symptoms observed. Store samples in a cool location until shipping.

Store at 4°C (refrigerator) if not shipping immediately

Do not freeze samples. This damages cell structure

Ship samples within 48 hours of collection

What to Avoid

✕Collecting wet material (wait until dew has dried)
✕Using dirty or rusty cutting tools
✕Mixing samples from different plants in one bag
✕Leaving samples in direct sunlight or hot vehicles
✕Freezing samples unless specifically instructed